User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/13: Difference between revisions
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* Ran kinetics on 150 uM solution (20uL ADA, 600uL 750 uM adenosine, and 2.3 8mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm. | * Ran kinetics on 150 uM solution (20uL ADA, 600uL 750 uM adenosine, and 2.3 8mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm. | ||
** dAbs was obtained and plotted vs. time in excel. | ** dAbs was obtained and plotted vs. time in excel. | ||
* Ran kinetics on 200 uM solution (20uL ADA, 600uL 1000 uM adenosine, and 2.3 8mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm. | * Ran kinetics on 200 uM solution (20uL ADA, 600uL 1000 uM adenosine, and 2.3 8mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm. | ||
** dAbs was obtained and plotted vs. time in excel. | ** dAbs was obtained and plotted vs. time in excel. | ||
* Average velocities were obtained. | |||
* The completion of trials 2 & 3 were followed by the calculation for the K<sub>m</sub> and V<sub>max</sub. Data was exchanged with Catherine Koenigsknecht and Michael Nagle. | |||
Revision as of 13:05, 13 February 2013
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Trial 2 & 3 of ADA Kinetic Assay
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