User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/19

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(Protocol)
(ADA Kinetic Assay with Acetylsalicylic Acid inhibitor)
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==ADA Kinetic Assay with Acetylsalicylic Acid inhibitor==
 
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* The objective for this laboratory period was to run ADA Kinetic assay with the addition of the inhibitor.
 
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* Acetylsalicylic acid (C<sub>9</sub>H<sub>8</sub>O<sub>4</sub>, MW 180.16) was obtained from Sigma-Aldrich, Co.
 
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* The assay of the acid is ≥ 99.0 %. The solid appeared as a white crystalline form having an absorbance of 230 nm.
 
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* The first trial conducted did not include the inhibitor for the purpose of obtaining a zero point.
 
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* There were observed inconsistencies among the 32, 39.7, 141.8, and 200 μM adenosine concentrations. Thus, new concentrations were calculated for adenosine.
 
==Protocol==
==Protocol==

Revision as of 17:17, 8 May 2013

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Protocol

  • The portion below was taken from the note book of Dhea Patel.
  • Image:Screen_Shot_2013-02-19_at_1.12.45_PM.png

Image:Screen_Shot_2013-02-19_at_2.46.05_PM.png

  • The data obtained from the ADA Assay was not consistent with previous findings, so new Adenosine concentrations were made up according to the following table:

Image:Screen_Shot_2013-02-19_at_3.19.14_PM.png

Data

Image:NOInhibitorAssay.tiff



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