User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/19

ADA Kinetic Assay with Acetylsalicylic Acid inhibitor

• The objective for this laboratory period was to run ADA Kinetic assay with the addition of the inhibitor.
• Acetylsalicylic acid (C₉H₈O₄, MW 180.16) was obtained from Sigma-Aldrich, Co.
• The assay of the acid is ≥ 99.0 %. The solid appeared as a white crystalline form having an absorbance of 230 nm.

• The first trial conducted did not include the inhibitor for the purpose of obtaining a zero point.
• There were observed inconsistencies among the 32, 39.7, 141.8, and 200 μM adenosine concentrations. Thus, new concentrations were calculated for adenosine.

Protocol

• The portion below was taken from the note book of User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/19 Dhea Patel.
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• The data obtained from the ADA Assay was not consistent with previous findings, so new Adenosine concentrations were made up according to the following table:

Data