User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/19

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

ADA Kinetic Assay with Acetylsalicylic Acid inhibitor

  • The objective for this laboratory period was to run ADA Kinetic assay with the addition of the inhibitor.
  • Acetylsalicylic acid (C9H8O4, MW 180.16) was obtained from Sigma-Aldrich, Co.
  • The assay of the acid is ≥ 99.0 %. The solid appeared as a white crystalline form having an absorbance of 230 nm.

  • The first trial conducted did not include the inhibitor for the purpose of obtaining a zero point.
  • There were observed inconsistencies among the 32, 39.7, 141.8, and 200 μM adenosine concentrations. Thus, new concentrations were calculated for adenosine.



  • The data obtained from the ADA Assay was not consistent with previous findings, so new Adenosine concentrations were made up according to the following table:




Personal tools