User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/20
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| + | ==Aspirin concentration for ADA Kinetic Assay== | ||
| + | * The assay for the concentrations below will be conducted next laboratory period. | ||
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| + | [[Image:Screen_Shot_2013-02-19_at_1.13.00_PM.png|center]] | ||
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==ADA Kinetic Assay for obtaining the zero point== | ==ADA Kinetic Assay for obtaining the zero point== | ||
* The objective of this laboratory period is to conduct adenosine deaminase (ADA) kinetic assay runs for the new calculated concentrations. | * The objective of this laboratory period is to conduct adenosine deaminase (ADA) kinetic assay runs for the new calculated concentrations. | ||
* UV 2550 Shimadzu spectrophotometer was baseline with 0.05 M sodium phosphate buffer. | * UV 2550 Shimadzu spectrophotometer was baseline with 0.05 M sodium phosphate buffer. | ||
| - | * The first | + | * The assays were prepared according to the data below. |
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| + | [[Image:Screen_Shot_2013-02-19_at_2.46.05_PM.png|center]] | ||
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| + | * After running the first trial, it was observed that the absorbance for 12.34 μM adenosine of trial 2 was superimposed over the 10.52 μM adenosine of trial 1. | ||
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| + | * It was suggested by Dr. Hartings to use Beer's Law for accurate measurement of the concentration of adenosine in solution. | ||
| + | * An article was provided by Dr. Hartings with the molar extinction coefficient, 1.53 x 10<sup>-4</sup> of adenosine at 260 nm. | ||
| + | * Thus, it was decided to run a full spectrum of adenosine at each given concentration specified above for accurate measurement of the concentration of adenosine. | ||
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| + | ==Data== | ||
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| + | [[Image:Concen1.png|center]] | ||
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| + | [[Image:AveVelo1.png|center]] | ||
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| + | [[Image:1adeno.png|center]] | ||
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| + | [[Image:Lin1.png|center]] | ||
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| + | [[Image:UV2.20.png|center]] | ||
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Revision as of 15:29, 22 February 2013
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Aspirin concentration for ADA Kinetic Assay
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