User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/20

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Revision as of 15:06, 20 February 2013

Project name

The objective of this laboratory period is to conduct adenosine deaminase (ADA) kinetic assay runs for the new calculated concentrations.
UV 2550 Shimadzu spectrophotometer was baseline with 0.05 M sodium phosphate buffer.
The assays were prepared according to the data below.

After running the first trial, it was observed that the absorbance for 12.34 μM adenosine of trial 2 was superimposed over the 10.52 μM adenosine of trial 1.

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		+	* After running the first trial, it was observed that the absorbance for 12.34 μM adenosine of trial 2 was superimposed over the 10.52 μM adenosine of trial 1.