User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/03/20: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 14: Line 14:
* Kaempferol trials were started.
* Kaempferol trials were started.
* After examining some of the data, it was concluded to adjust the 40 μM adenosine concentration to 25 μM.
* After examining some of the data, it was concluded to adjust the 40 μM adenosine concentration to 25 μM.
==Tables==
* The data below was taken from [[User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/03/20|Dhea Patel]].
*To measure the mass of Kaempferol and the volume of DMSO:
[[Image:Screen_Shot_2013-03-20_at_1.45.50_PM.png]]
*Table of volumes and concentrations:
[[Image:Kinetics_Histogram_table.tiff|px550]]


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 17:42, 22 March 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Histogram

  • It was decided to create a histogram for each inhibitor which includes the flavonoids and the non-nucleoside, acetylsalicylic acid analogs. Each inhibitor consisted of three trials.
  • From Sigma, 500 μM Kaempferol (C15H10O6, MW 286.24 g/mol) stock solution was prepared in dimethylsulfoxide (DMSO).
  • A kinetic assay run of 40 μM adenosine without inhibitors was executed through the kinetics of the UVProbe system.
  • After the three trials of 40 μM adenosine, aspirin trials were conducted.
  • The aspirin trials did not show inhibition. A possible conclusion for the inconsistency was that the aspirin stock solution has deteriorated.
  • Kaempferol trials were started.
  • After examining some of the data, it was concluded to adjust the 40 μM adenosine concentration to 25 μM.

Tables

  • The data below was taken from Dhea Patel.
  • To measure the mass of Kaempferol and the volume of DMSO:

  • Table of volumes and concentrations:

px550



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II/2013/03/20&oldid=685025"