User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/03/26

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==25 uM Adenosine Histogram Kinetic Assay Runs==
 
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* The following procedures and data were taken from [[User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/03/26|Dhea Patel]].
 
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==Preparing 500uM Acetylsalicylic Acid Stock Solution==
 
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*13.7mg acetylsalicylic acid was added to 506.957mL phosphate buffer:
 
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[[Image:Screen_Shot_2013-03-26_at_12.39.06_PM.png]]
 
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==Preparing 500uM 4-Acetoxybenzoic acid (Aldrich 248517-50G) Stock Solution==
 
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*4-Acetoxybenzoic acid (Aldrich 248517-50G) (180.17g/mol) [ZINC00225517]:
 
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*2.2mg 4-Acetoxybenzoic acid (Aldrich 248517-50G) was added to 24.4214mL phosphate buffer.
 
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*[[Image:Screen_Shot_2013-03-26_at_3.11.27_PM.png]]
 
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==Kinetics==
 
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*Data Table of Volumes and Concentrations Used
 
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[[Image:20130326_Data_Table.tiff|px200]]
 
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==Data==
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[[Image:20130326_Data_.tiff]]
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*The aspirin appears to be aiding the reaction rather than inhibiting the adenosine.
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