User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/03/27: Difference between revisions
From OpenWetWare
Mary Mendoza (talk | contribs) |
Mary Mendoza (talk | contribs) |
||
Line 10: | Line 10: | ||
* A stock solution of 5-hydroxy-2-methylbenzoic acid was prepared in DMSO. | * A stock solution of 5-hydroxy-2-methylbenzoic acid was prepared in DMSO. | ||
* A 500 μM stock solution of 3-methylacetylsalicylic acid was prepared in 0.05 M phosphate buffer. | * A 500 μM stock solution of 3-methylacetylsalicylic acid was prepared in 0.05 M phosphate buffer. | ||
* Assay for each of the mentioned compounds showed inhibition which can be seen on the data below. The data is provided by [[User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/03/27|Dhea Patel]]. | |||
==Preparing 500uM [Inhibitor] Solutions== | |||
*500uM [5-Hydroxy-2-methylbenzoic acid] (Aldrich 696366-1G) [ZINC01668680] made in DMSO: | |||
[[Image:Screen_Shot_2013-03-27_at_1.57.40_PM.png]] | |||
*500uM [3-Methylacetylsalicylic acid] (Aldrich S376647-1G) [ZINC00001221] made in Phosphate Buffer: | |||
[[Image:Screen_Shot_2013-03-27_at_2.49.54_PM.png]] | |||
==Data Table== | |||
[[Image:20130327_Data_Table.tiff]] | |||
* The table above shows the concentrations and volumes of the substrate and other chemical constituents present in the cuvette during data collection. | |||
* The kinetics was performed by the UVProbe of UV 2500 Shimadzu spectrophotometer at 25°C. | |||
==Data== | |||
[[Image:20130327_data.tiff]] | |||
* The histogram above shows that the 4-acetoxybenzoic acid shows the greatest inhibition as an aspirin analog. | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 13:19, 28 March 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Continuation of Histogram runs
Preparing 500uM [Inhibitor] Solutions
Data Table
Data
|