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Continuation of Histogram runs
- The final trial for 4-acetoxybenzoic acid was completed. This compound showed inhibition.
- A stock solution of 5-hydroxy-2-methylbenzoic acid was prepared in DMSO.
- A 500 μM stock solution of 3-methylacetylsalicylic acid was prepared in 0.05 M phosphate buffer.
- Assay for each of the mentioned compounds showed inhibition which can be seen on the data below. The data is provided by Dhea Patel.
Preparing 500uM [Inhibitor] Solutions
- 500uM [5-Hydroxy-2-methylbenzoic acid] (Aldrich 696366-1G) [ZINC01668680] made in DMSO:
- 500uM [3-Methylacetylsalicylic acid] (Aldrich S376647-1G) [ZINC00001221] made in Phosphate Buffer:
Data Table
- The table above shows the concentrations and volumes of the substrate and other chemical constituents present in the cuvette during data collection.
- The kinetics was performed by the UVProbe of UV 2500 Shimadzu spectrophotometer at 25°C.
Data
- The histogram above shows that the 4-acetoxybenzoic acid shows the greatest inhibition as an aspirin analog.
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