User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/04/03

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(Preparation of Reagents)
Current revision (18:32, 8 May 2013) (view source)
(Preparation of Reagents)
 
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==Preparation of Reagents==
 
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* 500 μM of each inhibitor was made with newly made 0.05 M phosphate buffer solution at the pH of 7.4.
 
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* A new stock solution of 200 μM adenosine stock solution was also prepared.
 
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* For the kinetic assay runs, it was decided to revert back to the original, chosen adenosine concentration of 40 μM.
 
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* Adenosine trials were performed at 265 nm. A histogram of that trial is shown below.
 
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[[Image:Pe2.png|center]]
 
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