User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/04/09: Difference between revisions
From OpenWetWare
Mary Mendoza (talk | contribs) |
Mary Mendoza (talk | contribs) |
||
Line 10: | Line 10: | ||
* Three runs of each inhibitor were taken; the average of the runs were taken. The standard deviation were calculated and added as error bars. | * Three runs of each inhibitor were taken; the average of the runs were taken. The standard deviation were calculated and added as error bars. | ||
* Produce histogram | * Produce histogram | ||
==Protocol== | |||
* The reagents were added in the following sequence: | |||
** 1.78 mL of phosphate buffer | |||
** 600 μL of 200 μM adenosine (final concentration 50 μM) | |||
* This was allowed to mix through venting and placed on the chamber for absorbance reading. | |||
** 300 μL of phosphate buffer (or inhibitor dissolved in phosphate buffer) | |||
** 300 μL of dimethyl sulfoxide (DMSO) (or inhibitor dissolved in DMSO) | |||
* The cuvette contents were mixed by venting and placed on the chamber for absorbance reading. | |||
** 20 μL of adenosine deaminase (ADA) was added to catalyze the reaction | |||
* The kinetics of the reaction was taken using the UVProbe software. | |||
==Description== | |||
*Cuvette Mixture with Inhibitor | |||
[[Image:InhibitorCuvetteTable.png]] | |||
*Cuvette Mixture no Inhibitor | |||
[[Image:CuvetteMixtureNoInhibitor.png]] | |||
*Inhibitors Ran today: | |||
#Aspirin | |||
#Kaempferol | |||
==Data== | |||
* [[Image:409HistogramTable.png]] | |||
* [[Image:409AverageVelocityHisto.png]] | |||
* [[Image:409PercentChangeHisto.png]] | |||
==Notes== | |||
*Solution Preparation/Calculations: Catherine Koenigsknecht | |||
*Cuvette preparation: Mary Mendoza | |||
*Data Evaluation: Dhea Patel | |||
*Overall Lab Assistance: Mike Nagle | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | |||
|} | |||
__NOTOC__ | |||
==Protocol== | ==Protocol== |
Revision as of 23:40, 11 April 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objective
Protocol
Description
DataNotes
|
Protocol
- The reagents were added in the following sequence:
- 1.78 mL of phosphate buffer
- 600 μL of 200 μM adenosine (final concentration 50 μM)
- This was allowed to mix through venting and placed on the chamber for absorbance reading.
- 300 μL of phosphate buffer (or inhibitor dissolved in phosphate buffer)
- 300 μL of dimethyl sulfoxide (DMSO) (or inhibitor dissolved in DMSO)
- The cuvette contents were mixed by venting and placed on the chamber for absorbance reading.
- 20 μL of adenosine deaminase (ADA) was added to catalyze the reaction
- The kinetics of the reaction was taken using the UVProbe software.
Description
- Cuvette Mixture with Inhibitor
- Cuvette Mixture no Inhibitor
- Inhibitors Ran today:
- Aspirin
- Kaempferol
Data
Notes
- Solution Preparation/Calculations: Catherine Koenigsknecht
- Cuvette preparation: Mary Mendoza
- Data Evaluation: Dhea Patel
- Overall Lab Assistance: Mike Nagle
|}