User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/04/09: Difference between revisions
From OpenWetWare
Mary Mendoza (talk | contribs) |
Mary Mendoza (talk | contribs) (→Notes) |
||
Line 87: | Line 87: | ||
*Data Evaluation: Dhea Patel | *Data Evaluation: Dhea Patel | ||
*Overall Lab Assistance: Mike Nagle | *Overall Lab Assistance: Mike Nagle | ||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 23:42, 11 April 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objective
Protocol
Description
DataNotes
|
Protocol
- The reagents were added in the following sequence:
- 1.78 mL of phosphate buffer
- 600 μL of 200 μM adenosine (final concentration 50 μM)
- This was allowed to mix through venting and placed on the chamber for absorbance reading.
- 300 μL of phosphate buffer (or inhibitor dissolved in phosphate buffer)
- 300 μL of dimethyl sulfoxide (DMSO) (or inhibitor dissolved in DMSO)
- The cuvette contents were mixed by venting and placed on the chamber for absorbance reading.
- 20 μL of adenosine deaminase (ADA) was added to catalyze the reaction
- The kinetics of the reaction was taken using the UVProbe software.
Description
- Cuvette Mixture with Inhibitor
- Cuvette Mixture no Inhibitor
- Inhibitors Ran today:
- Aspirin
- Kaempferol
Data
Notes
- Solution Preparation/Calculations: Catherine Koenigsknecht
- Cuvette preparation: Mary Mendoza
- Data Evaluation: Dhea Patel
- Overall Lab Assistance: Mike Nagle
|}