|
|
(6 intermediate revisions by the same user not shown) |
Line 6: |
Line 6: |
| | colspan="2"| | | | colspan="2"| |
| <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> |
| ==Objective==
| |
| * A run of 40 μM adenosine with 50 μM varying inhibitors were conducted using the UV 2500 Shimadzu spectrophotometer.
| |
| * Three runs of each inhibitor were taken; the average of the runs were taken. The standard deviation were calculated and added as error bars.
| |
| * Produce histogram
| |
|
| |
|
| ==Protocol==
| |
| * The reagents were added in the following sequence:
| |
| ** 1.78 mL of phosphate buffer
| |
| ** 600 μL of 200 μM adenosine (final concentration 50 μM)
| |
| * This was allowed to mix through venting and placed on the chamber for absorbance reading.
| |
| ** 300 μL of phosphate buffer (or inhibitor dissolved in phosphate buffer)
| |
| ** 300 μL of dimethyl sulfoxide (DMSO) (or inhibitor dissolved in DMSO)
| |
| * The cuvette contents were mixed by venting and placed on the chamber for absorbance reading.
| |
| ** 20 μL of adenosine deaminase (ADA) was added to catalyze the reaction
| |
| * The kinetics of the reaction was taken using the UVProbe software.
| |
|
| |
|
| *Cuvette Mixture no Inhibitor
| |
| [[Image:CuvetteMixtureNoInhibitor.png]]
| |
|
| |
|
| *Cuvette Mixture with Inhibitor
| |
| [[Image:CuvetteSolnInhibitorHisto.png]]
| |
|
| |
|
| *Inhibitors Ran today:
| |
| #4-Acetoxybenozic Acid [ZINC
| |
| #3',4',5,5',7-pentahydroxyflavone
| |
| #3,6,2',4',5-pentahydroxyflavone
| |
| #Datiscetin
| |
|
| |
|
| ==Data==
| |
| [[Image:PercentchangeHisot416.png|600px]]
| |
|
| |
|
| ==Notes==
| |
| *Solution Preparation/Calculations: Catherine Koenigsknecht
| |
| *Cuvette preparation: Mary Mendoza
| |
| *Data Evaluation: Dhea Patel
| |
| *Overall Lab Assistance: Mike Nagle
| |
|
| |
| <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
| |
| |}
| |
|
| |
| __NOTOC__
| |
|
| |
| ==Protocol==
| |
| * The reagents were added in the following sequence:
| |
| ** 1.78 mL of phosphate buffer
| |
| ** 600 μL of 200 μM adenosine (final concentration 50 μM)
| |
| * This was allowed to mix through venting and placed on the chamber for absorbance reading.
| |
| ** 300 μL of phosphate buffer (or inhibitor dissolved in phosphate buffer)
| |
| ** 300 μL of dimethyl sulfoxide (DMSO) (or inhibitor dissolved in DMSO)
| |
| * The cuvette contents were mixed by venting and placed on the chamber for absorbance reading.
| |
| ** 20 μL of adenosine deaminase (ADA) was added to catalyze the reaction
| |
| * The kinetics of the reaction was taken using the UVProbe software.
| |
|
| |
| *Cuvette Mixture no Inhibitor
| |
| [[Image:CuvetteMixtureNoInhibitor.png]]
| |
|
| |
| *Cuvette Mixture with Inhibitor
| |
| [[Image:CuvetteSolnInhibitorHisto.png]]
| |
|
| |
| *Inhibitors Ran today:
| |
| #4-Acetoxybenozic Acid [ZINC
| |
| #3',4',5,5',7-pentahydroxyflavone
| |
| #3,6,2',4',5-pentahydroxyflavone
| |
| #Datiscetin
| |
|
| |
| ==Data==
| |
| [[Image:PercentchangeHisot416.png|600px]]
| |
|
| |
| ==Notes==
| |
| *Solution Preparation/Calculations: Catherine Koenigsknecht
| |
| *Cuvette preparation: Mary Mendoza
| |
| *Data Evaluation: Dhea Patel
| |
| *Overall Lab Assistance: Mike Nagle
| |
|
| |
|
| <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |