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| ==Protocol==
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| * The reagents were added in the following sequence:
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| ** 1.78 mL of phosphate buffer
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| ** 600 μL of 200 μM adenosine (final concentration 50 μM)
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| * This was allowed to mix through venting and placed on the chamber for absorbance reading.
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| ** 300 μL of phosphate buffer (or inhibitor dissolved in phosphate buffer)
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| ** 300 μL of dimethyl sulfoxide (DMSO) (or inhibitor dissolved in DMSO)
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| * The cuvette contents were mixed by venting and placed on the chamber for absorbance reading.
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| ** 20 μL of adenosine deaminase (ADA) was added to catalyze the reaction
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| * The kinetics of the reaction was taken using the UVProbe software.
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| *Cuvette Mixture no Inhibitor
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| [[Image:CuvetteMixtureNoInhibitor.png]]
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| *Cuvette Mixture with Inhibitor
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| [[Image:CuvetteSolnInhibitorHisto.png]]
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| *Inhibitors Ran today:
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| #50uM [2,3-bis(actyloxy)benzoic aicd] [PH003446]
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| #50uM [Luteolin] [AMB18511398]
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| #50uM [Morin] [AMB18511704]
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| #50uM [Robinetin] [AMB18511408]
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| #50uM [5-Hydroxy-2-methylbenzoic acid] [Aldrich 696366-1G]
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| ==Data==
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| [[Image:417.png|600px]]
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| [[Image:410a.png|600px]]
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| ==Notes==
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| *Solution Preparation/Calculations: Catherine Koenigsknecht
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| *Cuvette preparation: Mary Mendoza
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| *Data Evaluation: Dhea Patel
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| *Overall Lab Assistance: Mike Nagle
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