|
|
| (2 intermediate revisions by the same user not shown) |
| Line 8: |
Line 8: |
|
| |
|
|
| |
|
| ==Protocol==
| |
| * The reagents were added in the following sequence:
| |
| ** 1.78 mL of phosphate buffer
| |
| ** 600 μL of 200 μM adenosine (final concentration 50 μM)
| |
| * This was allowed to mix through venting and placed on the chamber for absorbance reading.
| |
| ** 300 μL of phosphate buffer (or inhibitor dissolved in phosphate buffer)
| |
| ** 300 μL of dimethyl sulfoxide (DMSO) (or inhibitor dissolved in DMSO)
| |
| * The cuvette contents were mixed by venting and placed on the chamber for absorbance reading.
| |
| ** 20 μL of adenosine deaminase (ADA) was added to catalyze the reaction
| |
| * The kinetics of the reaction was taken using the UVProbe software.
| |
|
| |
|
| *Cuvette Mixture no Inhibitor
| |
| [[Image:CuvetteMixtureNoInhibitor.png]]
| |
|
| |
|
| *Cuvette Mixture with Inhibitor
| |
| [[Image:CuvetteSolnInhibitorHisto.png]]
| |
|
| |
|
| *Inhibitors Ran today:
| |
| #50uM [2,3-bis(actyloxy)benzoic aicd] [PH003446]
| |
| #50uM [Luteolin] [AMB18511398]
| |
| #50uM [Morin] [AMB18511704]
| |
| #50uM [Robinetin] [AMB18511408]
| |
| #50uM [5-Hydroxy-2-methylbenzoic acid] [Aldrich 696366-1G]
| |
|
| |
|
| ==Data==
| |
| [[Image:417.png|600px]]
| |
|
| |
|
|
| |
| [[Image:410a.png|600px]]
| |
|
| |
| ==Notes==
| |
| *Solution Preparation/Calculations: Catherine Koenigsknecht
| |
| *Cuvette preparation: Mary Mendoza
| |
| *Data Evaluation: Dhea Patel
| |
| *Overall Lab Assistance: Mike Nagle
| |
|
| |
|
|
| |
|
Project name
