User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/04/19: Difference between revisions
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* Stock solution of 200 μM inosine (MW 268.2 g/mol) was prepared. A spectrum was taken using the same dilution mentioned above. | * Stock solution of 200 μM inosine (MW 268.2 g/mol) was prepared. A spectrum was taken using the same dilution mentioned above. | ||
==Protocol== | ==Protocol for Assay== | ||
* The reagents were added in the following sequence: | * The reagents were added in the following sequence: | ||
** 1.78 mL of phosphate buffer | ** 1.78 mL of phosphate buffer | ||
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*Inhibitors Ran today: | *Inhibitors Ran today: | ||
# 50 μM 3-methylacetylsalicylic acid (ZINC 00001221)(Aldrich S376647-1G) | |||
# 50 μM Methyl (4-hydroxy-3-methoxyphenyl)acetate (Aldrich PH003466-1MG) | |||
# 50 μM [3,4-bis(acetyloxy)benzoic acid] (Aldrich PH00962-150MG) | |||
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Revision as of 22:02, 18 April 2013
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Objective
Procedure for Adenosine and Inosine Spectrum
Protocol for Assay
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