User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/08/29

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Preparation of Gold and Bovine Serum Albumin Stock Solutions

  • Using a metal spatula, Bovine Serum Albumin (BSA) was prepared by dissolving .0249 g of white crystalline powder of BSA into .025 L of water to obtain 15 μM of BSA.
  • The amount of .0249 g of BSA was acquired from the following calculation:

.025 L of water × (15×10-6 mM of BSA ÷ 1 L of water) = 3.75×10-7 mM of BSA

3.75×10-7 mM of BSA × 66430 g (formula weight of BSA) = .0249 g of BSA

  • Using a metal spatula, Gold (Au) stock solution was made by dissolving .085 g of hydrogen tetrachloroaurate (HAuCl4) in .025 L of water to obtain 10 mM HAuCl4.
  • The amount of .085 g of HAuCl4 was acquired from the following calculation:

.025 L of water × (10×10-3 mM of HAuCl4 ÷ 1L of water)= .00025 mM of HAuCl4

.00025 mM of HAuCl4 × 339. 79 g (formula weight of HAuCl4)= .085 g of HAuCl4

Preparation of different mole ratios of Au/BSA


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