User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/04: Difference between revisions

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3.82 × 10<sup>-7</sup> ÷ .025 L of water = 1.53 × 10<sup>-5</sup> mM/L
3.82 × 10<sup>-7</sup> ÷ .025 L of water = 1.53 × 10<sup>-5</sup> mM/L


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Revision as of 20:05, 14 September 2012

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UV-Vis Spectroscopy of Aug. 29 Au/BSA solutions

  • In reference to the entry of Wednesday, August 29 (a week ago), the Au and BSA solutions were made using a metal spatula. Due to its hygroscopic nature, HAuCl4 reacted with the metal spatula.
  • The Au/BSA solutions of Aug. 29 were still examined using UV-Vis. The values for each mole ratio were plotted by its intensity vs. time using MS Excel.

  • UV-visible scans of different Au/BSA mole ratios (batch August 29) incubated at 85 degrees Celsius for 4 h.


  • A closer view of the peaks of the UV-Visible scan of Au/BSA mole ratios from above.

Preparation of Au/BSA solutions

  • BSA solution was prepared by dissolving .0254 g of white crystalline powder of BSA in .025 L of water.

0.254 g of BSA × (1 mol of BSA ÷ 66430 g of BSA) = 3.82 × 10-7

3.82 × 10-7 ÷ .025 L of water = 1.53 × 10-5 mM/L


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