User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/04: Difference between revisions
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* Micropipets were used to transfer the Au/BSA solution from their test tube to the cuvette. | * Micropipets were used to transfer the Au/BSA solution from their test tube to the cuvette. | ||
* One single cuvette was used for all UV-Vis scans to avoid fluctuations in the UV-Vis scan readings. | * One single cuvette was used for all UV-Vis scans to avoid fluctuations in the UV-Vis scan readings. | ||
* During the UV-Vis scans, some of the fibers were stuck in the micropipet tips. These fibers were discarded. Hence, some of the volume of the Au/BSA solution were lost. | * During the UV-Vis scans, some of the fibers were stuck in the micropipet tips. These fibers were discarded. Hence, some of the volume of the Au/BSA solution were lost. The lost is negligible though. | ||
[[Image:AuBSAaug29edited.png]] | [[Image:AuBSAaug29edited.png]] |
Revision as of 20:47, 14 September 2012
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UV-Vis Spectroscopy of Aug. 29 Au/BSA solutions
Preparation of Au and BSA solutions
0.254 g of BSA × (1 mol of BSA ÷ 66430 g of BSA) = 3.82 × 10-7 3.82 × 10-7 ÷ .025 L of water = 1.53 × 10-5 mM/L
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