User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/04: Difference between revisions
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==Preparation of Tris Buffers== | ==Preparation of Tris Buffers== | ||
* Two tris buffers, pH 8 and 10, were made to test the effect of pH on the formation of AuNPs. | * Two tris buffers, pH 8 and 10, were made to test the effect of pH on the formation of AuNPs. | ||
* Both buffers were prepared to have a .1 M concentration. | * Both buffers were prepared to have a .1 M concentration. The tris buffer has a formula weight of 121.14 g. By adjusting the decimal point, it was verified that only 1.21 g is needed to be dissolved in 100 mL of water and obtain a .1 M concentration. | ||
* 1.213 g of white crystalline powder of tris buffer was dissolved into 85 mL of water. A pH probe was inserted in the tris buffer solution while stirring. | * 1.213 g of white crystalline powder of tris buffer was dissolved into 85 mL of water. A pH probe was inserted in the tris buffer solution while stirring. | ||
* HCl was gradually added to the tris buffer with the pH probe until the desired pH was reached. | * HCl was gradually added to the tris buffer with the pH probe until the desired pH was reached. 9 mL of HCl was added to the .1 M tris buffer to maintain pH 8. | ||
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UV-Vis Spectroscopy of Aug. 29 Au/BSA solutions
Preparation of Au and BSA solutions
0.254 g of BSA × (1 mol of BSA ÷ 66430 g of BSA) = 3.82 × 10-7 3.82 × 10-7 ÷ .025 L of water = 1.53 × 10-5 mM/L
0.0639 g of HAuCl4 × (1 mol ÷ 339.79 g) of HAuCl4 = 0.0001880 mol of HAuCl4 0.0001880 mol of HAuCl4 ÷ 0.025 L of water = 7522 μM of HAuCl4 Preparation of Tris Buffers
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