UV-Vis Spectroscopy of Aug. 29 Au/BSA solutions
- In reference to the entry of Wednesday, August 29 (a week ago), the Au and BSA solutions were made using a metal spatula. Due to its hygroscopic nature, HAuCl4 reacted with the metal spatula.
- The Au/BSA solutions of Aug. 29 were still examined using UV-Vis. The values for each mole ratio were plotted by its intensity vs. time using MS Excel.
- Micropipets were used to transfer the Au/BSA solution from their test tube to the cuvette.
- One single cuvette was used for all UV-Vis scans to avoid fluctuations in the UV-Vis scan readings.
- During the UV-Vis scans, some of the fibers were stuck in the micropipet tips. These fibers were discarded. Hence, some of the volume of the Au/BSA solution were lost.
- UV-visible scans of different Au/BSA mole ratios (batch August 29) incubated at 85 degrees Celsius for 4 h.
- A closer view of the peaks of the UV-Visible scan of Au/BSA mole ratios from above.
Preparation of Au and BSA solutions
- The metal spatulas used for preparation of stock solutions were wrapped with parafilm. This was done to inhibit HAuCl4 from reacting with the metal.
- BSA solution was prepared by dissolving .0254 g of white crystalline powder of BSA in .025 L of water.
0.254 g of BSA × (1 mol of BSA ÷ 66430 g of BSA) = 3.82 × 10-7
3.82 × 10-7 ÷ .025 L of water = 1.53 × 10-5 mM/L
- HAuCl4 stock solution was prepared by dissolving 0.0639 g of the orange, granular solid of HAuCl4 in .025 L of water.
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