User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/25: Difference between revisions
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* The membrane filter had a diameter of 47mm with a 450 nm pore diameter. | * The membrane filter had a diameter of 47mm with a 450 nm pore diameter. | ||
* The pump was turned on; the knob was unscrewed. The setting was set to 60 cmHg vacuum. This setting was chosen at random. | * The pump was turned on; the knob was unscrewed. The setting was set to 60 cmHg vacuum. This setting was chosen at random. | ||
* The first pour was done with the binding buffer. This was done to ensure that the buffer with the lower imidazole content (binding) would not be contaminated with the buffer that had the higher imidazole content (elution). | |||
Revision as of 02:23, 5 October 2012
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Lysis of transformed E. coli
Filtration of Buffers
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