User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/25: Difference between revisions
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* The collected filtrate of the binding buffer was poured into a sterilized glass bottle. This was followed by the pouring of the elution buffer into the filtering system. The filtrate for the elution buffer was also poured into a sterilized glass bottle. | * The collected filtrate of the binding buffer was poured into a sterilized glass bottle. This was followed by the pouring of the elution buffer into the filtering system. The filtrate for the elution buffer was also poured into a sterilized glass bottle. | ||
* The bottles were capped with aluminum foil tops and refrigerated. The buffers were clear, colorless liquid solution before and after filtration. | * The bottles were capped with aluminum foil tops and refrigerated. The buffers were clear, colorless liquid solution before and after filtration. | ||
* The filtrates were poured into their respective falcon tubes and refrigerated. | |||
==Filtration of the Supernatant== | ==Filtration of the Supernatant== |
Revision as of 02:42, 5 October 2012
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Lysis of transformed E. coli
Filtration of Buffers
Filtration of the Supernatant
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