User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/25: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Filtration of Buffers== | ==Filtration of Buffers== | ||
[[Image:Bufpix.jpg|thumb|right|Assembly of Membrane Filtration]] | [[Image:Bufpix.jpg|thumb|right|Assembly of Membrane Filtration Image Source: http://academic.pgcc.edu/~kroberts/Lecture/Chapter%206/counting.html]] | ||
* The assembly shown in the picture on the right was followed with an additional clip that would hold the filter tightly to the flask. The sidearm of the 1000 mL flask was connected to a Welch vacuum pump. | * The assembly shown in the picture on the right was followed with an additional clip that would hold the filter tightly to the flask. The sidearm of the 1000 mL flask was connected to a Welch vacuum pump. | ||
* The membrane filter had a diameter of 47mm with a 450 nm pore diameter. | * The membrane filter had a diameter of 47mm with a 450 nm pore diameter. | ||
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* The current pellet was left untouched in the centrifuge tubes. This are discarded material that contains disrupted cell organelles and other cellular material. | * The current pellet was left untouched in the centrifuge tubes. This are discarded material that contains disrupted cell organelles and other cellular material. | ||
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Latest revision as of 22:04, 26 September 2017
Project name | Main project page Previous entry Next entry |
Lysis of transformed E. coli
Filtration of Buffers
Filtration of the Supernatant
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