User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/25: Difference between revisions
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==Filtration of Buffers== | ==Filtration of Buffers== | ||
[[Image:Bufpix.jpg|thumb|right|Assembly of Membrane Filtration]] | [[Image:Bufpix.jpg|thumb|right|Assembly of Membrane Filtration Image Source: http://academic.pgcc.edu/~kroberts/Lecture/Chapter%206/counting.html]] | ||
* The assembly shown in the picture on the right was followed with an additional clip that would hold the filter tightly to the flask. The sidearm of the 1000 mL flask was connected to a Welch vacuum pump. | * The assembly shown in the picture on the right was followed with an additional clip that would hold the filter tightly to the flask. The sidearm of the 1000 mL flask was connected to a Welch vacuum pump. | ||
* The membrane filter had a diameter of 47mm with a 450 nm pore diameter. | * The membrane filter had a diameter of 47mm with a 450 nm pore diameter. |
Revision as of 06:38, 7 December 2012
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Lysis of transformed E. coli
Filtration of Buffers
Filtration of the Supernatant
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