User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/25: Difference between revisions
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==Lysis of transformed ''E. coli''== | ==Lysis of transformed ''E. coli''== | ||
* To retrieve the adenosine deaminase (ADA) from ''E.coli'', the cell must be lysed. By lysing the cell, the cytoplasm will no longer isolate the macromolecules, such as the ADA for this instance, from the extracellular environment. | * To retrieve the adenosine deaminase (ADA) from ''E.coli'', the cell must be lysed. By lysing the cell, the cytoplasm will no longer isolate the macromolecules, such as the ADA for this instance, from the extracellular environment. | ||
* There are different methods by which the cell can be lysed. These methods include heat shock, blender polytron, homogenization etc. For this procedure, | * There are different methods by which the cell can be lysed. These methods include heat shock, blender polytron, homogenization etc. For this procedure, sonication will be used to lyse the cell. | ||
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Revision as of 11:23, 25 September 2012
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Lysis of transformed E. coli
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