The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>
|
Lysis of transformed E. coli
- To retrieve the adenosine deaminase (ADA) from E.coli, the cell must be lysed. By lysing the cell, the cytoplasm will no longer isolate the macromolecules, such as the ADA for this instance, from the extracellular environment.
- There are different methods by which the cell can be lysed. These methods include heat shock, blender polytron, homogenization etc. For this procedure, sonication will be used to lyse the cell.
- By exposing the cells to high frequency sound waves of a sonicator, the cell membrane are sheared exposing the intracellular contents.
|
Project name
