User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02: Difference between revisions
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* Placed the settings of the spectrophotometer on kinetics, at 25°C, 510 nm from the methods function. | * Placed the settings of the spectrophotometer on kinetics, at 25°C, 510 nm from the methods function. | ||
* In reference to the document of [http://openwetware.org/wiki/Image:HRP_Assays.docx Novy, Patel, and Wang (MDK)], the group has chosen to prepare the sample solution with the most linearity. The sample solution has the following concentration of reactants listed in the table below. | * In reference to the document of [http://openwetware.org/wiki/Image:HRP_Assays.docx Novy, Patel, and Wang (MDK)], the group has chosen to prepare the sample solution with the most linearity. The sample solution has the following concentration of reactants listed in the table below. The original concentration of AAP was 2.5 mM amounting to 700 μL. | ||
* Transferred the reactants to a plastic disposable sample cell using a micropipet. NOTE: The HRP was added last when the sample was already inside the chamber. The addition of HRP initiates the reaction. | * Transferred the reactants to a plastic disposable sample cell using a micropipet. NOTE: The HRP was added last when the sample was already inside the chamber. The addition of HRP initiates the reaction. |
Revision as of 17:04, 8 October 2012
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Preparation of the Reactants of HRP Oxidase Assay
0.020 mol of Na2HPO4 × [math]\displaystyle{ \frac{268.07g}{1mol} }[/math] of Na2HPO4 = 53.61 g of Na2HPO4
HRP Assay Figures
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