User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02 - Revision history

Mary Mendoza: /* HRP Assay Figures */

Mary Mendoza — Tue, 09 Oct 2012 00:09:22 GMT

HRP Assay Figures

←Older revision		Revision as of 00:09, 9 October 2012
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	==HRP Assay Figures==		==HRP Assay Figures==
-	* Charts embedded below	+	* Charts embedded below represent the HRP Assay runs that have varying concentrations of HRP and AAP. The variations in the physical condition of the surrounding environment were done to investigate the appropriate ratio between HRP and AAP.
		+
	[[Image:HRP10022012.png]]		[[Image:HRP10022012.png]]

	* Runs 1, 3, and 4 reflect absorbance trends where the reaction had a low concentration of HRP.		* Runs 1, 3, and 4 reflect absorbance trends where the reaction had a low concentration of HRP.
	* Run 2 shows the reaction where there was an error in calculating the proper volume for the HRP and AAP ratio. The group added 50 μL of HRP against a very low concentration of AAP.		* Run 2 shows the reaction where there was an error in calculating the proper volume for the HRP and AAP ratio. The group added 50 μL of HRP against a very low concentration of AAP.
-	* Run 5 is the replicated absorbance from MDK using the ~~same conditions on~~ the ~~table above~~.	+	* Run 5 is the replicated absorbance from MDK using the standard condition where the volume of AAP is 700 μL and HRP is 50 μL.

	[[Image:DviewHRP10022012.png]]		[[Image:DviewHRP10022012.png]]
-

Mary Mendoza: /* Preparation of the Reactants of HRP Oxidase Assay */

Mary Mendoza — Tue, 09 Oct 2012 00:04:49 GMT

Preparation of the Reactants of HRP Oxidase Assay

←Older revision		Revision as of 00:04, 9 October 2012
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	* Placed the settings of the spectrophotometer on kinetics, at 25°C, 510 nm from the methods function.		* Placed the settings of the spectrophotometer on kinetics, at 25°C, 510 nm from the methods function.

-	* In reference to the document of [http://openwetware.org/wiki/Image:HRP_Assays.docx Novy, Patel, and Wang (MDK)], the group has chosen to prepare the sample solution with the most linearity. The sample solution has the following concentration of reactants listed in the table below.	+	* In reference to the document of [http://openwetware.org/wiki/Image:HRP_Assays.docx Novy, Patel, and Wang (MDK)], the group has chosen to prepare the sample solution with the most linearity. The sample solution has the following concentration of reactants listed in the table below. The original concentration of AAP was 2.5 mM amounting to 700 μL.

	* Transferred the reactants to a plastic disposable sample cell using a micropipet. NOTE: The HRP was added last when the sample was already inside the chamber. The addition of HRP initiates the reaction.		* Transferred the reactants to a plastic disposable sample cell using a micropipet. NOTE: The HRP was added last when the sample was already inside the chamber. The addition of HRP initiates the reaction.

Mary Mendoza: /* HRP Assay Figures */

Mary Mendoza — Mon, 08 Oct 2012 23:59:06 GMT

HRP Assay Figures

←Older revision		Revision as of 23:59, 8 October 2012
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	[[Image:DviewHRP10022012.png]]		[[Image:DviewHRP10022012.png]]
		+
		+
		+

	* The rates of runs 2 and 5 are shown below to indicate that the rate of run 5 (reaction with the proper amount of HRP) has a linear rate compared to run 2.		* The rates of runs 2 and 5 are shown below to indicate that the rate of run 5 (reaction with the proper amount of HRP) has a linear rate compared to run 2.

Mary Mendoza: /* HRP Assay Figures */

Mary Mendoza — Mon, 08 Oct 2012 23:58:20 GMT

HRP Assay Figures

←Older revision		Revision as of 23:58, 8 October 2012
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	[[Image:DviewHRP10022012.png]]		[[Image:DviewHRP10022012.png]]
		+
		+	* The rates of runs 2 and 5 are shown below to indicate that the rate of run 5 (reaction with the proper amount of HRP) has a linear rate compared to run 2.
		+
		+	[[Image:Ratesofrxnrun2&5.png]]
		+
		+	[[Image:Dviewratesofrxnrun2&5.png]]

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Mary Mendoza: /* HRP Assay Figures */

Mary Mendoza — Mon, 08 Oct 2012 23:05:35 GMT

HRP Assay Figures

←Older revision		Revision as of 23:05, 8 October 2012
Line 55:		Line 55:

	==HRP Assay Figures==		==HRP Assay Figures==
		+	* Charts embedded below
		+	[[Image:HRP10022012.png]]
		+
		+	* Runs 1, 3, and 4 reflect absorbance trends where the reaction had a low concentration of HRP.
		+	* Run 2 shows the reaction where there was an error in calculating the proper volume for the HRP and AAP ratio. The group added 50 μL of HRP against a very low concentration of AAP.
		+	* Run 5 is the replicated absorbance from MDK using the same conditions on the table above.
		+
		+	[[Image:DviewHRP10022012.png]]

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Mary Mendoza: /* Preparation of the Reactants of HRP Oxidase Assay */

Mary Mendoza — Mon, 08 Oct 2012 22:54:01 GMT

Preparation of the Reactants of HRP Oxidase Assay

←Older revision		Revision as of 22:54, 8 October 2012
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	* For the following day, the agenda would be to ascertain the appropriate volume of AAP while keeping the same concentration.		* For the following day, the agenda would be to ascertain the appropriate volume of AAP while keeping the same concentration.
-
-

	{\| {{table}}		{\| {{table}}

Mary Mendoza: /* Preparation of the Reactants of HRP Oxidase Assay */

Mary Mendoza — Mon, 08 Oct 2012 22:53:32 GMT

Preparation of the Reactants of HRP Oxidase Assay

←Older revision		Revision as of 22:53, 8 October 2012
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	\| Volume\|\|10 μL\|\| \|\|750 μL\|\|50 μL		\| Volume\|\|10 μL\|\| \|\|750 μL\|\|50 μL
	\|-		\|-
		+
		+	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
		+	\|}
		+
		+	__NOTOC__

	==HRP Assay Figures==		==HRP Assay Figures==

Mary Mendoza: /* Preparation of the Reactants of HRP Oxidase Assay */

Mary Mendoza — Mon, 08 Oct 2012 22:53:00 GMT

Preparation of the Reactants of HRP Oxidase Assay

←Older revision		Revision as of 22:53, 8 October 2012
Line 37:		Line 37:
	* For the following day, the agenda would be to ascertain the appropriate volume of AAP while keeping the same concentration.		* For the following day, the agenda would be to ascertain the appropriate volume of AAP while keeping the same concentration.

-	~~[[Image:HRP10022012.png]]~~	+

	{\| {{table}}		{\| {{table}}
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	\|-		\|-

-		+	==HRP Assay Figures==

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Mary Mendoza: /* Preparation of the Reactants of HRP Oxidase Assay */

Mary Mendoza — Mon, 08 Oct 2012 22:51:41 GMT

Preparation of the Reactants of HRP Oxidase Assay

←Older revision		Revision as of 22:51, 8 October 2012
Line 37:		Line 37:
	* For the following day, the agenda would be to ascertain the appropriate volume of AAP while keeping the same concentration.		* For the following day, the agenda would be to ascertain the appropriate volume of AAP while keeping the same concentration.

-		+	[[Image:HRP10022012.png]]

	{\| {{table}}		{\| {{table}}

Mary Mendoza: /* Preparation of the Reactants of HRP Oxidase Assay */

Mary Mendoza — Mon, 08 Oct 2012 05:36:22 GMT

Preparation of the Reactants of HRP Oxidase Assay

←Older revision		Revision as of 05:36, 8 October 2012
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	{\| {{table}}		{\| {{table}}
-	\| align="center" style="background:#f0f0f0;"\|'' ''	+	\| align="center" style="background:#f0f0f0;"\|''properties''
	\| align="center" style="background:#f0f0f0;"\|''4-iodophenol''		\| align="center" style="background:#f0f0f0;"\|''4-iodophenol''
	\| align="center" style="background:#f0f0f0;"\|''AAP''		\| align="center" style="background:#f0f0f0;"\|''AAP''

←Older revision		Revision as of 05:36, 8 October 2012
Line 40:		Line 40:

	{\| {{table}}		{\| {{table}}
-	\| align="center" style="background:#f0f0f0;"\|'' ''	+	\| align="center" style="background:#f0f0f0;"\|''properties''
	\| align="center" style="background:#f0f0f0;"\|''4-iodophenol''		\| align="center" style="background:#f0f0f0;"\|''4-iodophenol''
	\| align="center" style="background:#f0f0f0;"\|''AAP''		\| align="center" style="background:#f0f0f0;"\|''AAP''