User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/09: Difference between revisions
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* The sample cell used for this procedure is the ignited flame. | * The sample cell used for this procedure is the ignited flame. | ||
* A gold hollow cathode tube was selected for the procedure. The wavelength of the lamp was 242.8 nm. | * A gold hollow cathode tube was selected for the procedure. The wavelength of the lamp was 242.8 nm. | ||
* Two blanks were used; water and HCl. Water was chosen since it is part of the ternary mixture. The standards provided by [[User:Abigail E. Miller/Notebook/CHEM571 Lab Project/2012/10/08|Dr. Miller]] were made with HCl. In addition, HCl ionizes better and keeps the metal in solution. | * Two blanks were used; water and HCl. Water was chosen since it is part of the ternary mixture. The standards provided by [[User:Abigail E. Miller/Notebook/CHEM571 Lab Project/2012/10/08|Dr. Miller]] were made with HCl. In addition, HCl ionizes better and keeps the metal in solution. The standards were 5, 8, 10, 15, 20, 25, 30, 40 ppm Au which appeared as clear and colorless liquid solutions. | ||
* Each blank, water and HCl, was measured and followed by autozero. Next, standards 1, 2, and 3 were measured. | |||
* The flame is blue green in its normal state. Having run the blanks, the flame remained blue green during the re-measurement of each blank. | |||
Revision as of 15:04, 23 October 2012
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Atomic Absorption Spectroscopy of Au/BSA
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