User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/09

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Atomic Absorption Spectroscopy of Au/BSA

  • A rack of Au/BSA solutions was recovered and used for the assigned Atomic Absorption Spectroscopy (AAS). AAS was used to strip the molecule to its elemental state.
  • The entire solution set were observed to lack fibers even at high mole ratios where formation of fibers is typical. The date of which the solutions were made is unknown.
  • The equipment used for AAS is by the make of Shimadzu AA-6200 Atomic Absorption Flame Emission.
  • The sample cell used for this procedure is the ignited flame.
  • A gold hollow cathode tube was selected for the procedure. The wavelength of the lamp was 242.8 nm.
  • Two blanks were used; water and HCl. Water was chosen since it is part of the ternary mixture. The standards provided by User:Abigail E. Miller/Notebook/CHEM571 Lab Project/2012/10/08 Dr. Miller were made with HCl. In addition, HCl ionizes better and keeps the metal in solution.



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