User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/10

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(Background of Adenosine Deaminase)
Current revision (13:50, 17 October 2012) (view source)
(Primer Assignment)
 
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==Adenosine Deaminase==
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* Background and configuration of adenosine deaminase (ADA) were discussed by Dr. Costanzi.
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* A molecular visualization software, Maestro, was introduced.
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==Primer Assignment==
==Primer Assignment==
{| {{table}}
{| {{table}}
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| align="center" style="background:#f0f0f0;"|''properties''
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| align="center" style="background:#f0f0f0;"|''ADA E34A reverse primer''
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| align="center" style="background:#f0f0f0;"|''4-iodophenol''
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| align="center" style="background:#f0f0f0;"|''AAP''
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| align="center" style="background:#f0f0f0;"|''H<sub>2</sub>O<sub>2</sub>''
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| align="center" style="background:#f0f0f0;"|''HRP''
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|-
|-
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| Molarity||18 mM||156.25 μM||1.7 mM||2.3 μM
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| 5' GTT AAA TCT AAA TGG CAA TGT AAT GCA 3'
|-
|-
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| Volume||10 μL|| ||750 μL||50 μL
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| Tm = 62.9°C MW= 15, 067.8 g/mole
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|-
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| 14.6 OD = 260 nm
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|-
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| 30.4 nm = 0.46 mg
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|-
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Adenosine Deaminase

  • Background and configuration of adenosine deaminase (ADA) were discussed by Dr. Costanzi.
  • A molecular visualization software, Maestro, was introduced.

Primer Assignment

ADA E34A reverse primer
5' GTT AAA TCT AAA TGG CAA TGT AAT GCA 3'
Tm = 62.9°C MW= 15, 067.8 g/mole
14.6 OD = 260 nm
30.4 nm = 0.46 mg


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