User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/16: Difference between revisions
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* The procedure listed in [[AU Biomaterials Design Lab:Protocols/PCR Mutation Protocol|PCR Mutation protocol]] was strictly followed. The amplified DNA was contained in a sterilized, 1.5 microcentrifuge tube | * The procedure listed in [[AU Biomaterials Design Lab:Protocols/PCR Mutation Protocol|PCR Mutation protocol]] was strictly followed. After the addition of all reagents, the sample was placed in the thermocycler. The amplified DNA was contained in a sterilized, 1.5 microcentrifuge tube. | ||
==Continuation of Chemiluminescence== | ==Continuation of Chemiluminescence== | ||
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{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''' | |||
| align="center" style="background:#f0f0f0;"|''HRP'' | | align="center" style="background:#f0f0f0;"|''HRP'' | ||
| align="center" style="background:#f0f0f0;"|''4-iodophenol'' | | align="center" style="background:#f0f0f0;"|''4-iodophenol'' | ||
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| align="center" style="background:#f0f0f0;"|''H<sub>2</sub>O'' | | align="center" style="background:#f0f0f0;"|''H<sub>2</sub>O'' | ||
|- | |- | ||
| 33 μL||27 μL||450 μL||88 μL||902 μL | | Molarity of stock solutions||2.3 μM||18 mM||10 mM||1.7 mM||neutral | ||
|- | |||
| Volume||33 μL||27 μL||450 μL||88 μL||902 μL | |||
|- | |- | ||
Revision as of 07:00, 7 December 2012
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PCR mutation
0.46 mg = 0.46E6 ng
Continuation of Chemiluminescence
[math]\displaystyle{ \frac{1.91 g}{15 mL} }[/math] × [math]\displaystyle{ \frac{1 mol}{105.9784 g} }[/math] = [math]\displaystyle{ \frac{0.00120 mol}{mL} }[/math] × [math]\displaystyle{ \frac{1 mL}{1E(-3) L} }[/math] = [math]\displaystyle{ \frac{1.20 mol}{L} }[/math] = 1.20 M of sodium carbonate
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