User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/16: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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| colspan="2"| | | colspan="2"| | ||
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<math>\frac{0.46E6 ng}{x \mu L}</math> = <math>\frac{100 ng}{1 \mu L}</math> of primer in water = 4600 μL of water | <math>\frac{0.46E6 ng}{x \mu L}</math> = <math>\frac{100 ng}{1 \mu L}</math> of primer in water = 4600 μL of water | ||
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* The procedure listed in [[AU Biomaterials Design Lab:Protocols/PCR Mutation Protocol|PCR Mutation protocol]] was strictly followed. After the addition of all reagents, the sample was placed in the thermocycler. | * The procedure listed in [[AU Biomaterials Design Lab:Protocols/PCR Mutation Protocol|PCR Mutation protocol]] was strictly followed. After the addition of all reagents, the sample was placed in the thermocycler. The amplified DNA was contained in a sterilized, 1.5 microcentrifuge tube. | ||
==Continuation of Chemiluminescence== | ==Continuation of Chemiluminescence== | ||
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{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''' | |||
| align="center" style="background:#f0f0f0;"|''HRP'' | | align="center" style="background:#f0f0f0;"|''HRP'' | ||
| align="center" style="background:#f0f0f0;"|''4-iodophenol'' | | align="center" style="background:#f0f0f0;"|''4-iodophenol'' | ||
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| align="center" style="background:#f0f0f0;"|''H<sub>2</sub>O'' | | align="center" style="background:#f0f0f0;"|''H<sub>2</sub>O'' | ||
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| 33 μL||27 μL||450 μL||88 μL||902 μL | | Molarity of stock solutions||2.3 μM||18 mM||10 mM||1.7 mM||neutral | ||
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| Volume||33 μL||27 μL||450 μL||88 μL||902 μL | |||
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Latest revision as of 22:07, 26 September 2017
Project name | Main project page Previous entry Next entry | ||||||||||||||||||
PCR mutation
0.46 mg = 0.46E6 ng
Continuation of Chemiluminescence
[math]\displaystyle{ \frac{1.91 g}{15 mL} }[/math] × [math]\displaystyle{ \frac{1 mol}{105.9784 g} }[/math] = [math]\displaystyle{ \frac{0.00120 mol}{mL} }[/math] × [math]\displaystyle{ \frac{1 mL}{1E(-3) L} }[/math] = [math]\displaystyle{ \frac{1.20 mol}{L} }[/math] = 1.20 M of sodium carbonate
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