User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/16: Difference between revisions
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==Continuation of Chemiluminescence== | ==Continuation of Chemiluminescence== | ||
* The luminol prepared from the previous chemiluminescence laboratory period had a pH of 7 to 8. Reviewing a [http://openwetware.org/wiki/Image:PHofluminol.pdf journal article] of Xiaoyu, it was determined that the optimal pH of luminol was 12.5 As a result, it was decided to prepare a new solution of luminol at a pH of 10 to 11. | * The luminol prepared from the previous chemiluminescence laboratory period had a pH of 7 to 8. Reviewing a [http://openwetware.org/wiki/Image:PHofluminol.pdf journal article] of Xiaoyu, it was determined that the optimal pH of luminol was 12.5. As a result, it was decided to prepare a new solution of luminol at a pH of 10 to 11. | ||
* A weight of .0112 g of luminol was added to 6 mL of water. The buffer composed of a direct addition of .0737 g of sodium carbonate and .4358 g of sodium bicarbonate. | |||
* Using a pH meter, the electrode detected the pH at 8.73. As suggested by Dr. Hartings, a solution of sodium carbonate was made to increase the pH of the solution. | |||
* Several adjustments were made in increasing the pH. A total weight of 1.91 g of sodium carbonate dissolved in 15 mL of water was added to the 6 mL solution of luminol. | |||
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Revision as of 23:20, 25 October 2012
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PCR mutation
0.46 mg = 0.46E6 ng
Continuation of Chemiluminescence
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