User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/17
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(→Preparation of Lysozyme stock) 
(→Preparation of Lysozyme stock) 

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* Michael Nagle prepared a new set of Au/BSA solutions. However, calculations made are not available in his notebook.  * Michael Nagle prepared a new set of Au/BSA solutions. However, calculations made are not available in his notebook.  
* Lysozyme (14,307 Da) appeared as a white granular solid provided by Sigma. Knowing that 1 Da = 1 g/ mol, the following calculations were made to obtain a stock solution of 15.24 μM.  * Lysozyme (14,307 Da) appeared as a white granular solid provided by Sigma. Knowing that 1 Da = 1 g/ mol, the following calculations were made to obtain a stock solution of 15.24 μM.  
+  
.012 L of water × <math>\frac{15E(6)mol}{1 L}</math> = <math>\frac{1.80E(7)mol}{L}</math> × <math>\frac{14,307 g}{mol}</math> = 0.00258 g of lysozyme to obtain 15 μM  .012 L of water × <math>\frac{15E(6)mol}{1 L}</math> = <math>\frac{1.80E(7)mol}{L}</math> × <math>\frac{14,307 g}{mol}</math> = 0.00258 g of lysozyme to obtain 15 μM  
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* The amount to be measured is too small to be certain that the entire solid would be dissolved in water. As a result, a multiple of the calculated amount was chosen to be dissolved in water; the amount chosen was 0.0109 g. Once dissolved, this will be diluted to 15 μM.  * The amount to be measured is too small to be certain that the entire solid would be dissolved in water. As a result, a multiple of the calculated amount was chosen to be dissolved in water; the amount chosen was 0.0109 g. Once dissolved, this will be diluted to 15 μM.  
+  
0.0109 g of lysozyme × <math>\frac{1 mol}{14,307 g}</math> = 7.62E(7) mol ÷ .050 L of water = 1.524E(5) M = 15.24 μM  0.0109 g of lysozyme × <math>\frac{1 mol}{14,307 g}</math> = 7.62E(7) mol ÷ .050 L of water = 1.524E(5) M = 15.24 μM  
+  
+  * After preparing the stock, it was decided to prepare the Au/lysozyme solution next week. The clear and colorless, 15.24 μM stock solution of lysozyme was refrigerated.  
Revision as of 12:54, 27 October 2012
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Electrophoresis of the mutated DNA plasmids
UVVis for Chemiluminescence
Preparation of Lysozyme stock
