User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/17: Difference between revisions
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* Data collection was not possible. No signal was collected. | * Data collection was not possible. No signal was collected. | ||
==Preparation of Lysozyme | ==Preparation of Gold and Lysozyme stocks== | ||
* Michael Nagle prepared a new set of Au/BSA solutions. | * Michael Nagle prepared a new set of Au/BSA solutions. The stock concentration of BSA was 1.49 x 10<sup>-5</sup> mM/L and the stock concentration for Au was 8.24 mM. The mole ratios were kept the same as 60, 80, 100, 120, 128, 130, 132, 133, 134, 136, 138, 140, 160, and 170. Nagle finished the preparation of the set and placed the rack at 85°C for 4h. at the Thermo Scientific incubator. | ||
* Weighed out .014 g of monohydrate chloroauric acid and dissolved into 5 mL of water. The molarity of gold was calculated to be at 8240 μM. | |||
.014 g of gold × <math>\frac{1 mol}{339.79 g}</math> = 4.12E(-5) mol ÷ .005 L = <math>\frac{8.24E(-3)mol}{L}</math> = 8240 μM | |||
* Lysozyme (14,307 Da) appeared as a white granular solid provided by Sigma. Knowing that 1 Da = 1 g/ mol, the following calculations were made to obtain a stock solution of 15.24 μM. | * Lysozyme (14,307 Da) appeared as a white granular solid provided by Sigma. Knowing that 1 Da = 1 g/ mol, the following calculations were made to obtain a stock solution of 15.24 μM. | ||
Revision as of 07:18, 7 December 2012
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Electrophoresis of the mutated DNA plasmids
UV-Vis for Chemiluminescence
Preparation of Gold and Lysozyme stocks
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