User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/17

(Difference between revisions)

Revision as of 12:24, 27 October 2012

Project name

Transferred 5 μL of the mutated plasmid into a new sterilized tube; leaving out one tube containing 45 μL.
Simultaneously, added 1 μL of the gel loading dye 6x blue into the 5 μL plasmid and added 1 μL of DpnI into the 45 μL plasmid.
The sample containing the DpnI was placed in a VWR analog heatblock at 37°C.
The agarose gel was prepared by dissolving 1.23 g of agarose in 35 mL of TAE buffer. The agarose was heated in a microwave for 35s.
When the solution is clear, the agarose was poured into the gel electrophoresis tray with the comb in place.
A wait period of 30 min. was allotted for the gel to solidify. Upon observing the gel is already in its solidified state, the comb was gently removed to avoid breakage.

@@ Line 7: / Line 7: @@
 <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 ==Electrophoresis of the mutated DNA plasmids==
-* Insert content here...
+* Transferred 5 μL of the mutated plasmid into a new sterilized tube; leaving out one tube containing 45 μL.
+* Simultaneously, added 1 μL of the gel loading dye 6x blue into the 5 μL plasmid and added 1 μL of DpnI into the 45 μL plasmid.
+* The sample containing the DpnI was placed in a VWR analog heatblock at 37°C.
+* The agarose gel was prepared by dissolving 1.23 g of agarose in 35 mL of TAE buffer. The agarose was heated in a microwave for 35s.
+* When the solution is clear, the agarose was poured into the gel electrophoresis tray with the comb in place.
+* A wait period of 30 min. was allotted for the gel to solidify. Upon observing the gel is already in its solidified state, the comb was gently removed to avoid breakage.
+*