User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/17: Difference between revisions
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* The plasmid with the loading dye were inserted to the wells 3 and 4 using an automatic delivery pipet. | * The plasmid with the loading dye were inserted to the wells 3 and 4 using an automatic delivery pipet. | ||
* The Horton 5B Electrophoresis system (chamber containing the tray) was connected to the BioRad power supply powerpac 200 at 85V. | * The Horton 5B Electrophoresis system (chamber containing the tray) was connected to the BioRad power supply powerpac 200 at 85V. | ||
==UV-Vis for Chemiluminescence== | |||
* An attempt was made to use the UV-Vis equipment for chemiluminescence by blocking the source lamp (D-2). The measuring mode was set on energy setting. The widest slid width available was 5.0 nm. | |||
* Data collection was not possible. No signal was collected. | |||
Revision as of 10:15, 27 October 2012
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Electrophoresis of the mutated DNA plasmids
UV-Vis for Chemiluminescence
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