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Electrophoresis of the mutated DNA plasmids
- Transferred 5 μL of the mutated plasmid into a new sterilized tube; leaving out one tube containing 45 μL.
- Simultaneously, added 1 μL of the gel loading dye 6x blue into the 5 μL plasmid and added 1 μL of DpnI into the 45 μL plasmid.
- The sample containing the DpnI was placed in a VWR analog heatblock at 37°C.
- The agarose gel was prepared by dissolving 1.23 g of agarose in 35 mL of TAE buffer. The agarose was heated in a microwave for 35s.
- When the solution is clear, the agarose was poured into the gel electrophoresis tray with the comb in place.
- A wait period of 30 min. was allotted for the gel to solidify. Upon observing the gel is already in its solidified state, the comb was gently removed to avoid breakage.
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