User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/23 - Revision history

Mary Mendoza: /* Au/lysozyme solutions */

Mary Mendoza — Fri, 07 Dec 2012 14:55:45 GMT

Au/lysozyme solutions

←Older revision		Revision as of 14:55, 7 December 2012
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	==Au/lysozyme solutions==		==Au/lysozyme solutions==
-	* The calculations were supposed to be posted on the notebook of [[User:Michael F. Nagle/Notebook/Chem 571/2012/10/23\|Michael Nagle]].	+	* The calculations were supposed to be posted on the notebook of [[User:Michael F. Nagle/Notebook/Chem 571/2012/10/23\|Michael Nagle]].
		+	* The calculation the author had executed for stock solution preparation is on [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/17\|10/17/2012]]. The lysozyme stock solution was 15.24 μM and the gold was at 8.24 mM.
		+	* It was decided that the lysozyme would be kept at a 1.5 μM concentration for all sample varying the Au concentration. The same mole ratios from Au/BSA were used 60, 80, 100, 120, 128, 130, 132, 133, 134, 136, 138, 140, 160, and 170.
	* When the Au/lysozyme solutions were made, Nagle placed the rack in the Thermo Scientific incubator at 50°C for 4h.		* When the Au/lysozyme solutions were made, Nagle placed the rack in the Thermo Scientific incubator at 50°C for 4h.

Mary Mendoza: /* ADA preparation */

Mary Mendoza — Fri, 07 Dec 2012 14:49:55 GMT

ADA preparation

←Older revision		Revision as of 14:49, 7 December 2012
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	==ADA preparation==		==ADA preparation==
	* All ADA procedures for this lab period was conducted by [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/23\|Puja Mody]]. The same approach was conducted during [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/19\|09/19/2012]]		* All ADA procedures for this lab period was conducted by [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/23\|Puja Mody]]. The same approach was conducted during [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/19\|09/19/2012]]
		+	* This was a two day procedure continued by Michael Nagle on the following day, [[User:Michael F. Nagle/Notebook/Chem 571/2012/10/23\|10/23/2012]]
	* The main concept for the procedure: ''E. coli'' was transformed to express ADA. After a 4 h. protein expression on an incubator shaker, the solution were centrifuge. The supernatant was collected and stored since this contained the soluble ADA while the pellets (cellular debris) were discarded.		* The main concept for the procedure: ''E. coli'' was transformed to express ADA. After a 4 h. protein expression on an incubator shaker, the solution were centrifuge. The supernatant was collected and stored since this contained the soluble ADA while the pellets (cellular debris) were discarded.

Mary Mendoza: /* Au/lysozyme solutions */

Mary Mendoza — Fri, 07 Dec 2012 14:35:26 GMT

Au/lysozyme solutions

←Older revision		Revision as of 14:35, 7 December 2012
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	==Au/lysozyme solutions==		==Au/lysozyme solutions==
-	* The calculations were supposed to be posted on the notebook of [[User:Michael F. Nagle/Notebook/Chem 571/2012/10/23\|Michael Nagle]]. ~~This section will be updated as soon as additional information on Nagle would be added.~~	+	* The calculations were supposed to be posted on the notebook of [[User:Michael F. Nagle/Notebook/Chem 571/2012/10/23\|Michael Nagle]].
-		+
-		+
	* When the Au/lysozyme solutions were made, Nagle placed the rack in the Thermo Scientific incubator at 50°C for 4h.		* When the Au/lysozyme solutions were made, Nagle placed the rack in the Thermo Scientific incubator at 50°C for 4h.

Mary Mendoza: /* ADA preparation */

Mary Mendoza — Fri, 07 Dec 2012 14:31:29 GMT

ADA preparation

←Older revision		Revision as of 14:31, 7 December 2012
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	==ADA preparation==		==ADA preparation==
	* All ADA procedures for this lab period was conducted by [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/23\|Puja Mody]]. The same approach was conducted during [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/19\|09/19/2012]]		* All ADA procedures for this lab period was conducted by [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/23\|Puja Mody]]. The same approach was conducted during [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/19\|09/19/2012]]
-	* ''E. coli'' was transformed to express ADA.	+	* The main concept for the procedure: ''E. coli'' was transformed to express ADA. After a 4 h. protein expression on an incubator shaker, the solution were centrifuge. The supernatant was collected and stored since this contained the soluble ADA while the pellets (cellular debris) were discarded.

	==Au/lysozyme solutions==		==Au/lysozyme solutions==

Mary Mendoza: /* ADA preparation */

Mary Mendoza — Fri, 07 Dec 2012 14:28:06 GMT

ADA preparation

←Older revision		Revision as of 14:28, 7 December 2012
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	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==ADA preparation==		==ADA preparation==
-	* All ADA procedures for this lab period was conducted by [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/23\|Puja Mody]].	+	* All ADA procedures for this lab period was conducted by [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/23\|Puja Mody]]. The same approach was conducted during [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/19\|09/19/2012]]
		+	* ''E. coli'' was transformed to express ADA.

	==Au/lysozyme solutions==		==Au/lysozyme solutions==

Mary Mendoza: /* Au/lysozyme solutions */

Mary Mendoza — Sat, 27 Oct 2012 18:50:27 GMT

Au/lysozyme solutions

←Older revision		Revision as of 18:50, 27 October 2012
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	==Au/lysozyme solutions==		==Au/lysozyme solutions==
-	* The calculations were supposed to be posted on the notebook of [[User:Michael F. Nagle/Notebook/Chem 571/2012/10/23\|Michael Nagle]]. This section will be updated as soon as additional information on Nagle would be added.	+	* The calculations were supposed to be posted on the notebook of [[User:Michael F. Nagle/Notebook/Chem 571/2012/10/23\|Michael Nagle]]. This section will be updated as soon as additional information on Nagle would be added.
		+
		+
		+	* When the Au/lysozyme solutions were made, Nagle placed the rack in the Thermo Scientific incubator at 50°C for 4h.

	==AAS of Au/BSA solution of 10/17/12==		==AAS of Au/BSA solution of 10/17/12==

Mary Mendoza: /* AAS of Au/BSA solution of 10/17/12 */

Mary Mendoza — Sat, 27 Oct 2012 18:46:23 GMT

AAS of Au/BSA solution of 10/17/12

←Older revision		Revision as of 18:46, 27 October 2012
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	* The irregular amounts created difficulty for centrifugation. Hours was spent in balancing the liquid for each pair of tubes.		* The irregular amounts created difficulty for centrifugation. Hours was spent in balancing the liquid for each pair of tubes.
	* Time went by and the fibers formed pellets on the bottom of the tubes. The formation of pellets was acceptable to allow to take AAS of the solutions.		* Time went by and the fibers formed pellets on the bottom of the tubes. The formation of pellets was acceptable to allow to take AAS of the solutions.
-	* The Shimadzu	+	* The program for the Shimadzu AA-6200 Atomic Absorption Flame Emission was set on autozero. The flame was ignited exhibiting a blue-green color.
		+	* The blank used for the experiment was water.
		+	* The standards were run followed by an interval of sample-blank measurements. The calibration curve can be seen on the data section of [[User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/23\|Puja Mody's notebook.]]
		+

Mary Mendoza: /* AAS of Au/BSA solution of 10/17/12 */

Mary Mendoza — Sat, 27 Oct 2012 18:41:27 GMT

AAS of Au/BSA solution of 10/17/12

←Older revision		Revision as of 18:41, 27 October 2012
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	* Mole ratios 100 and others produced fibers, having solutions clear and colorless. The liquid was taken from each solution and transferred to falcon tubes; leaving some liquid suspending the fibers in the original tube. As a result, they were irregular amounts on each tube.		* Mole ratios 100 and others produced fibers, having solutions clear and colorless. The liquid was taken from each solution and transferred to falcon tubes; leaving some liquid suspending the fibers in the original tube. As a result, they were irregular amounts on each tube.
	* The irregular amounts created difficulty for centrifugation. Hours was spent in balancing the liquid for each pair of tubes.		* The irregular amounts created difficulty for centrifugation. Hours was spent in balancing the liquid for each pair of tubes.
-	* Time went by and the fibers formed pellets on the bottom of the tubes.	+	* Time went by and the fibers formed pellets on the bottom of the tubes. The formation of pellets was acceptable to allow to take AAS of the solutions.
		+	* The Shimadzu

Mary Mendoza: /* AAS of Au/BSA solution of 10/17/12 */

Mary Mendoza — Sat, 27 Oct 2012 18:39:59 GMT

AAS of Au/BSA solution of 10/17/12

←Older revision		Revision as of 18:39, 27 October 2012
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	==AAS of Au/BSA solution of 10/17/12==		==AAS of Au/BSA solution of 10/17/12==
-		+	* The Au/BSA solutions were taken out from the oven. Mole ratios 60, 80, and 120 were the only solutions that exhibited a consistent, clear purple color with no fibers. This was indicative that all gold nanoparticles were in solution and none have nucleated to BSA.
		+	* Mole ratios 100 and others produced fibers, having solutions clear and colorless. The liquid was taken from each solution and transferred to falcon tubes; leaving some liquid suspending the fibers in the original tube. As a result, they were irregular amounts on each tube.
		+	* The irregular amounts created difficulty for centrifugation. Hours was spent in balancing the liquid for each pair of tubes.
		+	* Time went by and the fibers formed pellets on the bottom of the tubes.

Mary Mendoza: /* AAS of Au/BSA solution of */

Mary Mendoza — Sat, 27 Oct 2012 18:31:25 GMT

AAS of Au/BSA solution of

←Older revision		Revision as of 18:31, 27 October 2012
Line 12:		Line 12:
	* The calculations were supposed to be posted on the notebook of [[User:Michael F. Nagle/Notebook/Chem 571/2012/10/23\|Michael Nagle]]. This section will be updated as soon as additional information on Nagle would be added.		* The calculations were supposed to be posted on the notebook of [[User:Michael F. Nagle/Notebook/Chem 571/2012/10/23\|Michael Nagle]]. This section will be updated as soon as additional information on Nagle would be added.

-	==AAS of Au/BSA solution of ==	+	==AAS of Au/BSA solution of 10/17/12==