User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/24: Difference between revisions
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* After the 30 sec. mark, the tubes were submerged in ice. This rapid change in temperatures is a method of heat shock. This is an adequate of method of allowing the cells to incorporate the mutated plasmid into their intracellular matrix. | * After the 30 sec. mark, the tubes were submerged in ice. This rapid change in temperatures is a method of heat shock. This is an adequate of method of allowing the cells to incorporate the mutated plasmid into their intracellular matrix. | ||
* Delivered 250 μL of the LB and SOC media using an automatic delivery pipet into the labeled tubes. | * Delivered 250 μL of the LB and SOC media using an automatic delivery pipet into the labeled tubes. | ||
* The tubes containing the media were centrifuge for 1 h. at 37°C by Dr. Miller. | |||
* | * | ||
Revision as of 12:07, 27 October 2012
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Transformation of BL21 cellsThe following procedures were conducted near the proximity of the Bunsen burner in accordance with aseptic techniques. The procedure is based from the laboratory Transformation protocol.
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