User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/11/13 - Revision history

Mary Mendoza: /* Beer's Law */

Mary Mendoza — Sun, 09 Dec 2012 18:18:19 GMT

Beer's Law

←Older revision		Revision as of 18:18, 9 December 2012
Line 92:		Line 92:
	* We can hypothesized that before running the UV-Vis scans for the kinetic assay of ADA with adenosine, adenosine will produce a more pronounced absorption and signal. Therefore, adenosine should be monitored at 235.		* We can hypothesized that before running the UV-Vis scans for the kinetic assay of ADA with adenosine, adenosine will produce a more pronounced absorption and signal. Therefore, adenosine should be monitored at 235.

-	* Adenosine is the substrate of ADA. As time passes, ADA catalyzes the formation of inosine from adenosine. Thus, the concentration of adenosine should decrease ~~per unit~~ time. When concentration of adenosine decreases, the absorbance monitored at 235 should also decrease ~~per unit~~ time.	+	* Adenosine is the substrate of ADA. As time passes, ADA catalyzes the formation of inosine from adenosine. Thus, the concentration of adenosine should decrease over time. When concentration of adenosine decreases, the absorbance monitored at 235 should also decrease over time.

	{\| {{table}}		{\| {{table}}

Mary Mendoza: /* UV-visible scans of Reagents */

Mary Mendoza — Sun, 09 Dec 2012 18:14:21 GMT

UV-visible scans of Reagents

←Older revision		Revision as of 18:14, 9 December 2012
Line 41:		Line 41:
	M<sub>2</sub> = .0307 mM		M<sub>2</sub> = .0307 mM

-	[[Image:~~SmallerAdenoino1113~~.png]]	+	[[Image:Adenoino1113labeled.png]]


Line 62:		Line 62:


-	[[Image:~~ADA~~.png]]	+	[[Image:ADAlabeled.png]]
-		+

	==Beer's Law==		==Beer's Law==

Mary Mendoza: /* Beer's Law */

Mary Mendoza — Sun, 09 Dec 2012 16:22:51 GMT

Beer's Law

←Older revision		Revision as of 16:22, 9 December 2012
Line 71:		Line 71:

	<math>\frac{\epsilon = A}{bc}</math>		<math>\frac{\epsilon = A}{bc}</math>
-

	* The calculation for the molar absorptivities of adenosine and inosine are shown below.		* The calculation for the molar absorptivities of adenosine and inosine are shown below.
Line 81:		Line 80:

	* At 265 <math>\frac{\epsilon = .4}{.0000307 M}</math> = 13029.32		* At 265 <math>\frac{\epsilon = .4}{.0000307 M}</math> = 13029.32
-

	For inosine		For inosine
-

	* At 235 <math>\frac{\epsilon = .14}{.0001 M}</math> = 1400		* At 235 <math>\frac{\epsilon = .14}{.0001 M}</math> = 1400

Mary Mendoza: /* Beer's Law */

Mary Mendoza — Sun, 09 Dec 2012 16:22:24 GMT

Beer's Law

←Older revision		Revision as of 16:22, 9 December 2012
Line 67:		Line 67:
	==Beer's Law==		==Beer's Law==
	* Wavelength 235 and 265 were monitored for the absorbance of the reagents adenosine and inosine Their absorbance are listed on the table below.		* Wavelength 235 and 265 were monitored for the absorbance of the reagents adenosine and inosine Their absorbance are listed on the table below.
-
-

	* By manipulating Beer's Law, the molar absorptivity was calculated from the absorbance and concentration of the substances.		* By manipulating Beer's Law, the molar absorptivity was calculated from the absorbance and concentration of the substances.
Line 80:		Line 78:

	* At 235 <math>\frac{\epsilon = .57}{.0000307 M}</math> = 18566.78		* At 235 <math>\frac{\epsilon = .57}{.0000307 M}</math> = 18566.78
-


Line 93:		Line 90:

	* At 265 <math>\frac{\epsilon = .6}{.0001 M}</math> = 6000		* At 265 <math>\frac{\epsilon = .6}{.0001 M}</math> = 6000
-
-


	* Evaluating the molar absorptivities for each wavelength, the results indicate that there is a greater difference of molar absorptivities between adenosine and inosine at wavelength 235. The molar absorptivity for adenosine was observed to be higher than inosine.		* Evaluating the molar absorptivities for each wavelength, the results indicate that there is a greater difference of molar absorptivities between adenosine and inosine at wavelength 235. The molar absorptivity for adenosine was observed to be higher than inosine.
-
-
-

	* We can hypothesized that before running the UV-Vis scans for the kinetic assay of ADA with adenosine, adenosine will produce a more pronounced absorption and signal. Therefore, adenosine should be monitored at 235.		* We can hypothesized that before running the UV-Vis scans for the kinetic assay of ADA with adenosine, adenosine will produce a more pronounced absorption and signal. Therefore, adenosine should be monitored at 235.
-
-
-

	* Adenosine is the substrate of ADA. As time passes, ADA catalyzes the formation of inosine from adenosine. Thus, the concentration of adenosine should decrease per unit time. When concentration of adenosine decreases, the absorbance monitored at 235 should also decrease per unit time.		* Adenosine is the substrate of ADA. As time passes, ADA catalyzes the formation of inosine from adenosine. Thus, the concentration of adenosine should decrease per unit time. When concentration of adenosine decreases, the absorbance monitored at 235 should also decrease per unit time.

		+	{\| {{table}}
		+	\| align="center" style="background:#f0f0f0;"\|''Wavelength''
		+	\| align="center" style="background:#f0f0f0;"\|''Adenosine''
		+	\| align="center" style="background:#f0f0f0;"\|''Inosine''
		+	\|-
		+	\| Absorbance at 235\|\|.57\|\|.14
		+	\|-
		+	\| Absorbance at 265\|\|.4\|\|.6
		+	\|-

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Mary Mendoza: /* Beer's Law */

Mary Mendoza — Sun, 09 Dec 2012 16:21:23 GMT

Beer's Law

←Older revision		Revision as of 16:21, 9 December 2012
Line 68:		Line 68:
	* Wavelength 235 and 265 were monitored for the absorbance of the reagents adenosine and inosine Their absorbance are listed on the table below.		* Wavelength 235 and 265 were monitored for the absorbance of the reagents adenosine and inosine Their absorbance are listed on the table below.

-	~~{\| {{table}}~~	+
-	~~\| align="center" style="background:#f0f0f0;"\|''Wavelength''~~	+
-	~~\| align="center" style="background:#f0f0f0;"\|''Adenosine''~~	+
-	~~\| align="center" style="background:#f0f0f0;"\|''Inosine''~~	+
-	\|-	+
-	~~\| Absorbance at 235\|\|.57\|\|.14~~	+
-	\|-	+
-	~~\| Absorbance at 265\|\|.4\|\|.6~~	+
-	\|-	+

	* By manipulating Beer's Law, the molar absorptivity was calculated from the absorbance and concentration of the substances.		* By manipulating Beer's Law, the molar absorptivity was calculated from the absorbance and concentration of the substances.

Mary Mendoza: /* Beer's Law */

Mary Mendoza — Sun, 09 Dec 2012 16:21:06 GMT

Beer's Law

←Older revision		Revision as of 16:21, 9 December 2012
Line 101:		Line 101:

	* At 265 <math>\frac{\epsilon = .6}{.0001 M}</math> = 6000		* At 265 <math>\frac{\epsilon = .6}{.0001 M}</math> = 6000
		+



	* Evaluating the molar absorptivities for each wavelength, the results indicate that there is a greater difference of molar absorptivities between adenosine and inosine at wavelength 235. The molar absorptivity for adenosine was observed to be higher than inosine.		* Evaluating the molar absorptivities for each wavelength, the results indicate that there is a greater difference of molar absorptivities between adenosine and inosine at wavelength 235. The molar absorptivity for adenosine was observed to be higher than inosine.
		+
		+


	* We can hypothesized that before running the UV-Vis scans for the kinetic assay of ADA with adenosine, adenosine will produce a more pronounced absorption and signal. Therefore, adenosine should be monitored at 235.		* We can hypothesized that before running the UV-Vis scans for the kinetic assay of ADA with adenosine, adenosine will produce a more pronounced absorption and signal. Therefore, adenosine should be monitored at 235.
		+
		+

Mary Mendoza: /* Beer's Law */

Mary Mendoza — Sun, 09 Dec 2012 16:20:40 GMT

Beer's Law

←Older revision		Revision as of 16:20, 9 December 2012
Line 105:		Line 105:

	* Evaluating the molar absorptivities for each wavelength, the results indicate that there is a greater difference of molar absorptivities between adenosine and inosine at wavelength 235. The molar absorptivity for adenosine was observed to be higher than inosine.		* Evaluating the molar absorptivities for each wavelength, the results indicate that there is a greater difference of molar absorptivities between adenosine and inosine at wavelength 235. The molar absorptivity for adenosine was observed to be higher than inosine.
		+

	* We can hypothesized that before running the UV-Vis scans for the kinetic assay of ADA with adenosine, adenosine will produce a more pronounced absorption and signal. Therefore, adenosine should be monitored at 235.		* We can hypothesized that before running the UV-Vis scans for the kinetic assay of ADA with adenosine, adenosine will produce a more pronounced absorption and signal. Therefore, adenosine should be monitored at 235.
		+

	* Adenosine is the substrate of ADA. As time passes, ADA catalyzes the formation of inosine from adenosine. Thus, the concentration of adenosine should decrease per unit time. When concentration of adenosine decreases, the absorbance monitored at 235 should also decrease per unit time.		* Adenosine is the substrate of ADA. As time passes, ADA catalyzes the formation of inosine from adenosine. Thus, the concentration of adenosine should decrease per unit time. When concentration of adenosine decreases, the absorbance monitored at 235 should also decrease per unit time.

Mary Mendoza: /* Beer's Law */

Mary Mendoza — Sun, 09 Dec 2012 16:20:12 GMT

Beer's Law

←Older revision		Revision as of 16:20, 9 December 2012
Line 108:		Line 108:
	* We can hypothesized that before running the UV-Vis scans for the kinetic assay of ADA with adenosine, adenosine will produce a more pronounced absorption and signal. Therefore, adenosine should be monitored at 235.		* We can hypothesized that before running the UV-Vis scans for the kinetic assay of ADA with adenosine, adenosine will produce a more pronounced absorption and signal. Therefore, adenosine should be monitored at 235.

		+	* Adenosine is the substrate of ADA. As time passes, ADA catalyzes the formation of inosine from adenosine. Thus, the concentration of adenosine should decrease per unit time. When concentration of adenosine decreases, the absorbance monitored at 235 should also decrease per unit time.

Mary Mendoza: /* Beer's Law */

Mary Mendoza — Sun, 09 Dec 2012 16:11:42 GMT

Beer's Law

←Older revision		Revision as of 16:11, 9 December 2012
Line 102:		Line 102:
	* At 265 <math>\frac{\epsilon = .6}{.0001 M}</math> = 6000		* At 265 <math>\frac{\epsilon = .6}{.0001 M}</math> = 6000

		+
		+
		+	* Evaluating the molar absorptivities for each wavelength, the results indicate that there is a greater difference of molar absorptivities between adenosine and inosine at wavelength 235. The molar absorptivity for adenosine was observed to be higher than inosine.
		+
		+	* We can hypothesized that before running the UV-Vis scans for the kinetic assay of ADA with adenosine, adenosine will produce a more pronounced absorption and signal. Therefore, adenosine should be monitored at 235.

Mary Mendoza: /* Beer's Law */

Mary Mendoza — Sun, 09 Dec 2012 16:05:30 GMT

Beer's Law

←Older revision		Revision as of 16:05, 9 December 2012
Line 88:		Line 88:

	* At 235 <math>\frac{\epsilon = .57}{.0000307 M}</math> = 18566.78		* At 235 <math>\frac{\epsilon = .57}{.0000307 M}</math> = 18566.78
		+


	* At 265 <math>\frac{\epsilon = .4}{.0000307 M}</math> = 13029.32		* At 265 <math>\frac{\epsilon = .4}{.0000307 M}</math> = 13029.32
		+

	For inosine		For inosine
		+

	* At 235 <math>\frac{\epsilon = .14}{.0001 M}</math> = 1400		* At 235 <math>\frac{\epsilon = .14}{.0001 M}</math> = 1400
		+

	* At 265 <math>\frac{\epsilon = .6}{.0001 M}</math> = 6000		* At 265 <math>\frac{\epsilon = .6}{.0001 M}</math> = 6000