User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/11/14: Difference between revisions
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==ADA Kinetic Assay Runs== | ==ADA Kinetic Assay Runs== | ||
* During the previous lab entry, the molar absorptivities of adenosine and inosine were calculated. Adenosine was found to have a higher molar absorptivity at 235. | * During the previous lab entry, the molar absorptivities of adenosine and inosine were calculated. Adenosine was found to have a higher molar absorptivity at 235. | ||
* It was concluded from the previous period that the absorption of adenosine should decrease over time. A kinetic assay scan of the reaction containing 2.7 mL of sodium phosphate buffer, 300 μL of adenosine, and 15 μL of ADA was taken. | * It was concluded from the previous period that the absorption of adenosine should decrease over time. A kinetic assay scan of the reaction containing 2.7 mL of 0.05 M sodium phosphate buffer, 300 μL of 3.07 mM adenosine, and 15 μL of 42 μM ADA was taken. | ||
* The program was set to Kinetics with the wavelength at 235 for a collection span of 600s. | * The program was set to Kinetics with the wavelength at 235 for a collection span of 600s. | ||
Revision as of 12:09, 9 December 2012
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ADA Kinetic Assay Runs
UV-vis of Adenosine and Inosine |