User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/11/14

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ADA Kinetic Assay Runs

  • During the previous lab entry, the molar absorptivities of adenosine and inosine were calculated. Adenosine was found to have a higher molar absorptivity at 235.
  • It was concluded from the previous period that the absorption of adenosine should decrease over time. A UV-vis scan of a reaction containing 2.7 mL of sodium phosphate buffer, 300 μL of adenosine, and 15 μL of ADA was taken.




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