User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/11/27

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(Kinetics of ADA)
(Kinetics of ADA)
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|''Volume of ADA (μL)''
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| align="center" style="background:#f0f0f0;"|''Concentration of ADA (μM)''
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| align="center" style="background:#f0f0f0;"|''Volume of phosphate buffer (mL)''
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| align="center" style="background:#f0f0f0;"|''Concentration of phosphate buffer (M)''
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| align="center" style="background:#f0f0f0;"|''Volume of adenosine (μL)''
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| align="center" style="background:#f0f0f0;"|''Concentration of adenosine (mM)''
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| 15||0.21||2.95||0.0492||50||0.0512
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| 25||0.35||2.95||0.0492||50||0.0512
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| 40||0.56||2.95||0.0492||50||0.0512
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| 50||0.70||2.95||0.0492||50||0.0512
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Revision as of 16:45, 9 December 2012

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Dialysis Results

  • Dialysis tubes were prepared for adenosine deaminase (ADA) fractions 2 and 8 on 11/14/12. The ADA were removed from the dialysis tubes.
  • ADA fraction 2 appeared clear and colorless with no signs of precipitation.
  • ADA fraction 8 precipitated out of solution.

Kinetics of ADA

  • The activity of ADA was measured by monitoring the absorbance of inosine over time.

Image:AbsoofIIno.png


Volume of ADA (μL) Concentration of ADA (μM) Volume of phosphate buffer (mL) Concentration of phosphate buffer (M) Volume of adenosine (μL) Concentration of adenosine (mM)
150.212.950.0492500.0512
250.352.950.0492500.0512
400.562.950.0492500.0512
500.702.950.0492500.0512


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