User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/11/27: Difference between revisions
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==Kinetics of ADA== | ==Kinetics of ADA== | ||
* The activity of ADA was measured by monitoring the absorbance of inosine. | * The activity of ADA was measured by monitoring the absorbance of inosine over time. | ||
[[Image:AbsoofIIno.png]] | |||
* The trends among runs 1, 2, and 3 seem consistent. The increase of the concentration of the enzyme with each addition increases the rate of production of inosine with respect to time. | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|''Volume of ADA (μL)'' | |||
| align="center" style="background:#f0f0f0;"|''Concentration of ADA (μM)'' | |||
| align="center" style="background:#f0f0f0;"|''Volume of phosphate buffer (mL)'' | |||
| align="center" style="background:#f0f0f0;"|''Concentration of phosphate buffer (M)'' | |||
| align="center" style="background:#f0f0f0;"|''Volume of adenosine (μL)'' | |||
| align="center" style="background:#f0f0f0;"|''Concentration of adenosine (mM)'' | |||
|- | |||
| 15||0.21||2.95||0.0492||50||0.0512 | |||
|- | |||
| 25||0.35||2.95||0.0492||50||0.0512 | |||
|- | |||
| 40||0.56||2.95||0.0492||50||0.0512 | |||
|- | |||
| 50||0.70||2.95||0.0492||50||0.0512 | |||
|- | |||
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Revision as of 15:47, 9 December 2012
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Dialysis Results
Kinetics of ADA
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