User:Mary Mendoza/Notebook/CHEM 581: Experimental Chemistry I/2014/08/29: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Continuation of Film Synthesis== | ||
* | * Following the protocol provided by [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2014/08/29|Dr. Hartings]], the films from last Friday, [[User:Mary Mendoza/Notebook/CHEM 581: Experimental Chemistry I/2014/08/27|August 27, 2014]], were transferred from the teflon dishes into a 250 mL beaker containing 0.2 M Sodium Sulfate solution with added sulfuric acid at 80°C. | ||
* After an hour of gentle stirring (2:00PM), the films were transferred to a warmed, 1% HCl solution at 80°C. | |||
* The PVA only film was torn into pieces by the stir bar during stirring while the PVA + clay film remained intact. | |||
* At 2:35PM, the films were transferred to a room temperature, 0.2M Sodium Bicarbonate solution for 15 minutes. As mentioned, this process will neutralize the acid present on the films. | |||
* Once the 15 minute neutralization was completed, the films were stored into separate beakers containing distilled water for the meantime. | |||
==Batch 2 Film Synthesis== | |||
* 0.1144 g of Sodium Cloisite (Southern Clay Products) was added to 1.0068 g of PVA (MP Biomedicals); these were dissolved in 7 mL of distilled water in preparation for polymer + clay film synthesis. | |||
* PVA only film was prepared by dissolving 1.0091 g of PVA into 7 mL of distilled water. | |||
* The polymer solutions were stirred and heated at 75°C until mixture was homogeneous. | |||
* Observing no particles were left in solution, the polymer solutions were allowed to cool at room temperature. Once cooled, 0.5 mL of 0.8% glutaraldehyde was added to each polymer solution. Additional stirring was done on both solutions to ensure homogeneity. | |||
* Solutions were cast into their respected teflon dishes and left to dry under the fume hood. | |||
==Suspension of 8/27/14 Films in Malachite Green== | |||
* 2, 8, 80, and 200 ppm solutions of malachite green were prepared. The calculations were executed by [[User:James C. Schwabacher/Notebook/CHEM-571/2014/08/29|James Schwabacher]]. The smaller concentrations were made from diluting the stock solutions; 8, 80, and 200 ppm. [[User:Matt Hartings|Matt Hartings]] <b>How were these solutions prepared??? Who prepared them?</b> | |||
* Empty vials were labeled, accordingly, with the prepared concentrations of the malachite green. | |||
* The polymer films temporarily stored in distilled water were transferred into separate petri dishes for cutting. | |||
* Using a falcon tube and a metal spatula, the polymer films were cut into rounds. | |||
* The pieces of polymer film were distributed to the empty vials. The mass of the vial containing films were recorded in the table below: | |||
[[Image:Vials 8.27 films.png|center]] | |||
* 10 mL of the prepared malachite green solutions were added to the corresponding, labeled vials. | |||
Latest revision as of 00:13, 27 September 2017
Project name | Main project page Previous entry Next entry |
Continuation of Film Synthesis
Batch 2 Film Synthesis
Suspension of 8/27/14 Films in Malachite Green
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