User:Mary Mendoza/Notebook/CHEM 581: Experimental Chemistry I/2014/10/01: Difference between revisions

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* Using a vernier caliper, the thickness of the PVA-clay film used for diffusion is 0.51 mm.
* Using a vernier caliper, the thickness of the PVA-clay film used for diffusion is 0.51 mm.
* The diffusion assembly was prepared according to the picture below, containing 10 mL of 20 ppm Malachite green on the left chamber and 10 mL of distilled, deionized water:
* The diffusion assembly was prepared according to the picture below, containing 10 mL of 20 ppm Malachite green on the left chamber and 10 mL of distilled, deionized water:
[[Image:10 1 MG Diffusion1 916AM.JPG|thumb|600x600px|center]]
[[Image:10 1 MG Diffusion1 916AM.JPG|thumb|600x600px|center|Status at 9:16 AM]]
[[Image:10 1 MG Diffusion2 916AM.JPG|thumb|600x600px|center]]
[[Image:10 1 MG Diffusion2 916AM.JPG|thumb|600x600px|center|Status at 9:16 AM]]
 
* 10 uL of the 20 ppm Malachite green stock was diluted into 200 uL of DI water. This mixture was transferred into a 200 uL quartz, fluorescence cuvette; the absorbance was measured as the zero time for the diffusion curve. The absorbance of 200 uL of DI water was directly measured as the zero mark for the diffusion curve.
* At the 5 minute mark, 10 uL was taken from each chamber and placed in an eppendorf tube; this was diluted to 200 uL of DI water.
* We measured the UV-Vis spectrum for both chambers using a 200 μL cuvette. We returned each to the chamber it originally came from
* Measurements were taken for the following times
** 15 minutes
** 30 minutes
** 60 minutes
** 120 minutes
** 150 minutes
 


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Malachite Green Diffusion through PVA-clay film

  • I prepared 20 ppm malachite green from a 195 ppm malachite green stock solution provided by Dr. Hartings.
  • Using dilution equation, the volume of 195 ppm of malachite green required to make 25 mL of 20 ppm was calculated to be 2.56 mL.
  • Using a vernier caliper, the thickness of the PVA-clay film used for diffusion is 0.51 mm.
  • The diffusion assembly was prepared according to the picture below, containing 10 mL of 20 ppm Malachite green on the left chamber and 10 mL of distilled, deionized water:
Status at 9:16 AM
Status at 9:16 AM
  • 10 uL of the 20 ppm Malachite green stock was diluted into 200 uL of DI water. This mixture was transferred into a 200 uL quartz, fluorescence cuvette; the absorbance was measured as the zero time for the diffusion curve. The absorbance of 200 uL of DI water was directly measured as the zero mark for the diffusion curve.
  • At the 5 minute mark, 10 uL was taken from each chamber and placed in an eppendorf tube; this was diluted to 200 uL of DI water.
  • We measured the UV-Vis spectrum for both chambers using a 200 μL cuvette. We returned each to the chamber it originally came from
  • Measurements were taken for the following times
    • 15 minutes
    • 30 minutes
    • 60 minutes
    • 120 minutes
    • 150 minutes



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