User:Mary Mendoza/Notebook/CHEM 581: Experimental Chemistry I/2014/10/24

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Column Chromatography for BPA Concentration

  • The column used was a 30 cc plastic syringe. The first layer was filled with glass wool fiber and fine sand. The sand was ground by Dr. Hartings using mortar and pestle.
  • 9.9999 g of BDH Silica Gel 60 Angstroms High Purity 40-63 μm was mixed with approximately 30 mL of methanol.
  • First, the column was washed with water. Afterwards, the silica-methanol mixture was slowly poured to leave the sand layer undisturbed.
  • After some of the solvent front, methanol, passed, the column was washed with water again. Air pressure was applied to compact the column.



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